sars cov 2 b 1 117 grna (ATCC)

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ATCC sars cov 2 b 1 117 grna
Positive controls used in the study for SARS-CoV-2 targets and variants, Gene blocks are dsDNA molecules with the target sequence purchased from Integrated DNA Technologies (IDT, Coralville, Iowa).

Positive controls used in the study for SARS-CoV-2 targets and variants, Gene blocks are dsDNA molecules with the target sequence purchased from Integrated DNA Technologies (IDT, Coralville, Iowa).

Sars Cov 2 B 1 117 Grna, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sars+cov+2+grna/Heat-inactivated+SARS-CoV-2+variant+B%2E1%2E1%2E7/pmc11487133-5-14-17
Average 93 stars, based on 19 article reviews

sars cov 2 b 1 117 grna - by Bioz Stars, 2026-10

93/100 stars

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1) Product Images from "Human pathogen nucleic acids in wastewater solids from 191 wastewater treatment plants in the United States"

Article Title: Human pathogen nucleic acids in wastewater solids from 191 wastewater treatment plants in the United States

Journal: Scientific Data

doi: 10.1038/s41597-024-03969-8

Figure Legend Snippet: Positive controls used in the study for SARS-CoV-2 targets and variants, Gene blocks are dsDNA molecules with the target sequence purchased from Integrated DNA Technologies (IDT, Coralville, Iowa).

Techniques Used: Sequencing, Positive Control, Mutagenesis, Control, Blocking Assay

Figure Legend Snippet: Forward and reverse primers, and probe sequences for detection of SARS-CoV-2 nucleic acids in this study.

Techniques Used: Sequencing

Polymerase Chain Reaction:

Article Title: West Nile Virus ( Orthoflavivirus nilense ) RNA concentrations in wastewater solids at five wastewater treatment plants in the United States
Article Snippet: .. PCR positive controls consisted of synthetic viral WNV gRNA (VR-3274SD, ATCC) and SARS-CoV-2 gRNA (VR-1986D, ATCC). ddRT-PCR was performed on 20 μl samples from a 22 μl reaction volume, prepared using 5.5 μl template, mixed with 5.5 μl of One-Step RT-ddPCR Advanced Kit for Probes (Bio-Rad 1863021), 2.2 μl of 200 U/μl Reverse Transcriptase, 1.1 μl of 300 mM dithiothreitol (DDT) and primers and probes mixtures at a final concentration of 900 nM and 250 nM respectively. .. Primer and probes for assays were purchased from Integrated DNA Technologies (IDT, San Diego, CA) (Table 2).

Article Title: West Nile Virus ( Orthoflavivirus nilense ) RNA concentrations in wastewater solids at five wastewater treatment plants in the United States
Article Snippet: .. PCR positive controls consisted of synthetic viral WNV gRNA (VR-3274SD, ATCC) and SARS-CoV-2 gRNA (VR-1986D, ATCC). ddRT-PCR was performed on 20 μl samples from a 22 μl reaction volume, prepared using 5.5 μl template, mixed with 5.5 μl of One-Step RT-ddPCR Advanced Kit for Probes (1863021; Bio-Rad, Hercules, CA, USA), 2.2 μl of 200 U/μl Reverse Transcriptase, 1.1 μl of 300 mM dithiothreitol (DDT) and primers and probes mixtures at a final concentration of 900 nM and 250 nM respectively. .. Primer and probes for assays were purchased from Integrated DNA Technologies (IDT, San Diego, CA) ( ).

Reverse Transcription:

Article Title: West Nile Virus ( Orthoflavivirus nilense ) RNA concentrations in wastewater solids at five wastewater treatment plants in the United States
Article Snippet: .. PCR positive controls consisted of synthetic viral WNV gRNA (VR-3274SD, ATCC) and SARS-CoV-2 gRNA (VR-1986D, ATCC). ddRT-PCR was performed on 20 μl samples from a 22 μl reaction volume, prepared using 5.5 μl template, mixed with 5.5 μl of One-Step RT-ddPCR Advanced Kit for Probes (1863021; Bio-Rad, Hercules, CA, USA), 2.2 μl of 200 U/μl Reverse Transcriptase, 1.1 μl of 300 mM dithiothreitol (DDT) and primers and probes mixtures at a final concentration of 900 nM and 250 nM respectively. .. Primer and probes for assays were purchased from Integrated DNA Technologies (IDT, San Diego, CA) ( ).

Concentration Assay:

Article Title: West Nile Virus ( Orthoflavivirus nilense ) RNA concentrations in wastewater solids at five wastewater treatment plants in the United States
Article Snippet: .. PCR positive controls consisted of synthetic viral WNV gRNA (VR-3274SD, ATCC) and SARS-CoV-2 gRNA (VR-1986D, ATCC). ddRT-PCR was performed on 20 μl samples from a 22 μl reaction volume, prepared using 5.5 μl template, mixed with 5.5 μl of One-Step RT-ddPCR Advanced Kit for Probes (1863021; Bio-Rad, Hercules, CA, USA), 2.2 μl of 200 U/μl Reverse Transcriptase, 1.1 μl of 300 mM dithiothreitol (DDT) and primers and probes mixtures at a final concentration of 900 nM and 250 nM respectively. .. Primer and probes for assays were purchased from Integrated DNA Technologies (IDT, San Diego, CA) ( ).

Sequencing:

Article Title: Nationwide wastewater sequencing surveillance of SARS-CoV-2 lineages: validation against clinical data across 28 U.S. states
Article Snippet: .. For all sequencing runs, SARS-CoV-2 gRNA (ATCC VR-1986D), an early isolate of the original SARS-CoV-2 virus from March of 2020 in Washington, is used as a positive control (GenBank: MT246667.1 ). ..

Virus:

Positive Control:

Blocking Assay:

Article Title: Human viral nucleic acids concentrations in wastewater solids from Central and Coastal California USA
Article Snippet: SARS-CoV-2 BA_4_ORF1a_Del_141143 , ORF1a , SARS-CoV-2 gRNA (ATCC VR-1986D), Twist Synthetic gRNA for Omicron BA.1, Omicron BA.2, and Delta , Gene block. .. SARS-CoV-2 BA_2_75_S_147E_S_152R , S gene , SARS-CoV-2 gRNA (ATCC VR-1986D, Twist Synthetic gRNA for Omicron BA.1, Omicron BA.2 , Gene block. ..

Article Title: Human viral nucleic acids concentrations in wastewater solids from Central and Coastal California USA
Article Snippet: SARS-CoV-2 S gene (rev 2) , S gene , SARS-CoV-2 gRNA (ATCC VR-1986D), Twist Synthetic gRNA for Delta, Mu, Lambda, Beta , Twist Synthetic gRNA for Omicron BA.1. .. SARS-CoV-2 BA_4_ORF1a_Del_141143 , ORF1a , SARS-CoV-2 gRNA (ATCC VR-1986D), Twist Synthetic gRNA for Omicron BA.1, Omicron BA.2, and Delta , Gene block. .. SARS-CoV-2 BA_2_75_S_147E_S_152R , S gene , SARS-CoV-2 gRNA (ATCC VR-1986D, Twist Synthetic gRNA for Omicron BA.1, Omicron BA.2 , Gene block.

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